RESUMO
AIMS: This study was conducted to isolate and identify propionate-producing bacteria that can be used as an inoculum in improving wet brewers grains and rumen fermentation via increasing propionate concentration. METHODS AND RESULTS: A strain of Lactobacillus that exhibits high levels of propionate production was identified and characterized as Lactobacillus mucosae 521129 by 16S rRNA gene sequencing and phylogenetic analyses. Wet brewers grains were fermented through L. mucosae inoculation and resulted in an increase in propionate concentration. Fermented wet brewers grains were used in in vitro rumen fermentation and revealed that L. mucosae-fermented wet brewers grains produced more gas and had higher accumulations propionate and total volatile fatty acid (VFA) than the control. The fewest methanogen DNA copies were detected in L. mucosae-fermented wet brewers grains. CONCLUSION: Identified L. mucosae improved the fermentation of wet brewers grains and the in vitro rumen fermentation via increasing propionate and total VFA concentrations. SIGNIFICANCE AND IMPACT OF THE STUDY: The presented research provided the identification of L. mucosae 521129 as a propionate producer and was metabolically profiled. Furthermore, data present the putative application of this organism in improving the fermentation of wet brewers grains and in vitro rumen fermentation.
RESUMO
La inyección directa de silicona para aumentar el tamañode las mamas se asocia a importantes efectos adversos. Sepresenta un caso de una paciente de 47 años que había recibidomúltiples inyecciones de líquido de silicona 13 años antes yque acudió a la consulta de mamografía para el estudio descreening mamario. Los hallazgos en la mamografía y en laecografía fueron típicos de granulomas de silicona. El diagnósticoprecoz de cáncer de mama en estos pacientes es complicadodebido a que los cambios asociados a la inyección de siliconapueden simular el aspecto de la neoplasia e interfir en lacorrecta interpretación de los estudios radiológicos
Injection of liquid silicone for breast augmentation is associated to development of important adverse effects. We present a case of a 47-year-old woman with a history of liquid silicone injection 13 years before who underwent screening study. Mammographic and sonographic appearances were classic of silicone granulomas. Early detection of breast cancer in these patients is difficult due to architectural distortion associated to silicone injection that mimics neoplasm and interfere in the correct interpretation of radiological studies(AU)
